Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75373

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

View all CXCL12/SDF-1 alpha ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat SDF-1(alpha) in samples. No significant cross-reactivity or interference between Rat SDF-1(alpha) and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.05%

Inter-Assay Precision (Precision between assays) CV% < 5.11%

Recovery for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

Recovery

85-107%

Linearity

Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

Scientific Data Images for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

ELISA: Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric) [NBP2-75373] -

ELISA: Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric) [NBP2-75373] - Standard Curve Reference

Kit Contents for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CXCL12/SDF-1 alpha

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Alternate Names

SDF1 alpha, SDF1a

Gene Symbol

CXCL12

Additional CXCL12/SDF-1 alpha Products

Product Documents for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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