We have received an inquiry from our customer who is interested in your product, IL8 ELISA Kit (Catalog #NBP1-92706). Could you tell us the whether the product contains monkey-derived component? Is it Monkey IL-8 Standard Concentrate?

Name: Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-92706

Yes, this kit includes Monkey IL-8 Standard Concentrate (125000 U/ml) which will be used for creating the standard curve. The standard curve range for IL8 can be created from 19.5 to 1,250 U/mL and this kit has been suggested for IL8 detection with sensitivity limits of as little as 1.5 U/mL.

Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92706

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (baboon, cynomolgus) (50 uL)

Sensitivity

3.0 U/mL

Assay Range

19.5 - 1,250 U/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Monkey

Description

The monkey IL-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey IL-8

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.3%

Inter-Assay Precision (Precision between assays) CV% < 8.7%

Scientific Data Images for Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

ELISA: Monkey CXCL8/IL-8 ELISA Kit (Colorimetric) [NBP1-92706]

ELISA: CXCL8/IL-8 ELISA Kit [Biotin] [NBP1-92706] - Standard curve

Kit Contents for Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

Adhesive Plate Covers
Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey IL-8, Biotin-Conjugate (anti-IL-8 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
Aluminium pouch(es) with a monkey IL-8 Standard curve (coloured)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-8/CXCL8

Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8. Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes. The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Gene Symbol

CXCL8

Additional IL-8/CXCL8 Products

Product Documents for Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download Manual - Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

COA

SDS

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Product Specific Notices for Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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