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Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06757

Novus Biologicals, part of Bio-Techne
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NBP3-06757

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rabbit

Specificity

This kit recognizes Rabbit CXCL8/IL-8 in samples. No significant cross-reactivity or interference between Rabbit CXCL8/IL-8 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 4.98

Inter-Assay Precision (Precision between assays) %CV < 5.82

Recovery for Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

Recovery

91 - 109%

Linearity

Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

Scientific Data Images for Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

ELISA: Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric) [NBP3-06757] -

ELISA: Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric) [NBP3-06757] - Standard Curve Reference

Kit Contents for Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-8/CXCL8

IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Gene Symbol

CXCL8

Additional IL-8/CXCL8 Products

Product Documents for Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit CXCL8/IL-8 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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