Human Cystatin E/M/CST6 ELISA Kit (Colorimetric)

Name: Human Cystatin E/M/CST6 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-80316

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80316

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

1.51 pg/ml

Assay Range

7.8-500 pg/ml (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Specificity

Recognizes both recombinant and natural Human Cystatin E/M/CST6.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.83

Inter-Assay Precision (Precision between assays) CV% < 3.13

Recovery for Human Cystatin E/M/CST6 ELISA Kit (Colorimetric)

Recovery

110-116%

Scientific Data Images for Human Cystatin E/M/CST6 ELISA Kit (Colorimetric)

ELISA: Human Cystatin E/M/CST6 ELISA Kit (Colorimetric) [NBP2-80316]

ELISA: Human Cystatin E/M/CST6 ELISA Kit (Colorimetric) [NBP2-80316] - Example standard curve for Human Cystatin E/M/CST6 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human Cystatin E/M/CST6 ELISA Kit (Colorimetric)

1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: Cystatin E/M

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype.

Alternate Names

CST6

Gene Symbol