Human DNA Polymerase gamma ELISA Kit (Colorimetric)

Name: Human DNA Polymerase gamma ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39426

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39426

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.123 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human DNA Polymerase gamma ELISA Kit (Colorimetric)

ELISA: Human DNA Polymerase gamma ELISA Kit (Colorimetric) [NBP3-39426]

ELISA: Human DNA Polymerase gamma ELISA Kit (Colorimetric) [NBP3-39426] - Standard Curve Reference

Kit Contents for Human DNA Polymerase gamma ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: DNA Polymerase gamma

As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil-provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.

Long Name

DNA polymerase subunit gamma-1

Alternate Names

MDP1, POLG, PolG-alpha, POLG1, POLGA

Gene Symbol

POLG

Additional DNA Polymerase gamma Products

Product Documents for Human DNA Polymerase gamma ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human DNA Polymerase gamma ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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