Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68114

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

View all EN-RAGE/S100A12 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.67%

Inter-Assay Precision (Precision between assays) CV% < 10.58%

Recovery for Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

Recovery

89-113%

Linearity

Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

ELISA: Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence) [NBP2-68114] -

ELISA: Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence) [NBP2-68114] - Standard Curve Reference

Kit Contents for Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: EN-RAGE/S100A12

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. S100A12 is also a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B--a key transcription factor for inflammatory events--is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.

Long Name

Extracellular Newly Identified RAGE-binding Protein

Alternate Names

CAAF1, CAAFI, CAGC, CAGCS100, Calgranulin C, ENRAGE, MRP6, S100A12

Gene Symbol

S100A12

Additional EN-RAGE/S100A12 Products

Product Documents for Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human EN-RAGE/S100A12 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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