Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26057

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

36 ng/mL

Assay Range

72 ng/mL - 2500 ng/mL

View all ENPP-2/Autotaxin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse ENPP-2/Autotaxin. No significant cross-reactivity or interference between mouse ENPP-2/Autotaxin and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Recovery for Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric)

Recovery

83 - 102%

Scientific Data Images for Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric)

Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric)

Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric) [NBP3-26057] -

Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric) [NBP3-26057] - Standard Curve Reference

Kit Contents for Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Antibody
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ENPP-2/Autotaxin

The protein encoded by the ENPP2 gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2

Alternate Names

ATX, Autotaxin, ENPP2, Lysophosphatidic Acid, NPP2, PDNP2

Gene Symbol

ENPP2

Additional ENPP-2/Autotaxin Products

Product Documents for Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse ENPP-2/Autotaxin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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