Human ERK3/MAPK6 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00379

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all ERK3 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Human ERK3/MAPK6 in samples. No significant cross-reactivity or interference between Human Human ERK3/MAPK6 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.79%

Inter-Assay Precision (Precision between assays) 4.66%

Recovery for Human ERK3/MAPK6 ELISA Kit (Colorimetric)

Recovery

90 - 103%

Linearity

Human ERK3/MAPK6 ELISA Kit (Colorimetric)

Scientific Data Images for Human ERK3/MAPK6 ELISA Kit (Colorimetric)

Human ERK3/MAPK6 ELISA Kit (Colorimetric)

ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] -

ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] - Standard Curve Reference

Kit Contents for Human ERK3/MAPK6 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ERK3

ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4).

Long Name

Extracellular Signal-regulated Kinase 3

Alternate Names

MAPK6, p97MAPK

Gene Symbol

MAPK6

Additional ERK3 Products

Product Documents for Human ERK3/MAPK6 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ERK3/MAPK6 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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