Human Factor IX ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60518

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell Culture, CSF

Sensitivity

0.4 ng/mL (example only; lot dependent)

Assay Range

1.56 - 100 ng/mL; 0.313 - 20 mIU/mL (example only; lot dependent)

View all Coagulation Factor IX ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 3.50 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 8.90 (example only; lot dependent)

Recovery for Human Factor IX ELISA Kit (Colorimetric)

Recovery

99.00% (example only; lot dependent)

Scientific Data Images for Human Factor IX ELISA Kit (Colorimetric)

ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518]

ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518]

ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.
ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518]

ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518]

ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.

Kit Contents for Human Factor IX ELISA Kit (Colorimetric)

  • Biotinylated Human Factor IX Antibody (50X) - 120 uL
  • Chromogen Substrate (1X) - 80 uL
  • EIA Diluent (10X) - 30 mL
  • Human Factor IX microplate - 12 strips of 8 wells
  • Human Factor IX Standard - 80 ng
  • Negative Control - 1 vial
  • Plate sealing tapes - 3 EA
  • Positive Control - 1 vial
  • SP Conjugate (100X) - 80 uL
  • Stop Solution (1X) - 12 mL
  • Wash Buffer (20X) - 2 x 30 mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: Coagulation Factor IX

Factor IX encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Alternate Names

Christmas Factor, F9, FIX, HEMB, P19, Plasma Thromboplastic Component, PTC, THPH8

Gene Symbol

F9

Additional Coagulation Factor IX Products

Product Documents for Human Factor IX ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Factor IX ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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