Rat Factor IX ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82482

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Coagulation Factor IX ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Factor IX in samples. No Significant cross-reactivity or interference between Rat Factor IX and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.52

Inter-Assay Precision (Precision between assays) CV% < 5.39

Recovery for Rat Factor IX ELISA Kit (Colorimetric)

Recovery

88-106%

Linearity

Rat Factor IX ELISA Kit (Colorimetric)

Scientific Data Images for Rat Factor IX ELISA Kit (Colorimetric)

Rat Factor IX ELISA Kit (Colorimetric)

ELISA: Rat Factor IX ELISA Kit (Colorimetric) [NBP2-82482] -

ELISA: Rat Factor IX ELISA Kit (Colorimetric) [NBP2-82482] - Standard Curve Reference

Kit Contents for Rat Factor IX ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor IX

Factor IX encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Alternate Names

Christmas Factor, F9, FIX, HEMB, P19, Plasma Thromboplastic Component, PTC, THPH8

Gene Symbol

F9

Additional Coagulation Factor IX Products

Product Documents for Rat Factor IX ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Factor IX ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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