Rat Factor VIII ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82481

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

4.69 pg/mL

Assay Range

7.81 - 500 pg/mL

View all Factor VIII ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Factor VIII in samples. No Significant cross-reactivity or interference between Rat Factor VIII and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.5

Inter-Assay Precision (Precision between assays) CV% < 5.64

Recovery for Rat Factor VIII ELISA Kit (Colorimetric)

Recovery

84-104%

Linearity

Rat Factor VIII ELISA Kit (Colorimetric)

Scientific Data Images for Rat Factor VIII ELISA Kit (Colorimetric)

Rat Factor VIII ELISA Kit (Colorimetric)

ELISA: Rat Factor VIII ELISA Kit (Colorimetric) [NBP2-82481] -

ELISA: Rat Factor VIII ELISA Kit (Colorimetric) [NBP2-82481] - Standard Curve Reference

Kit Contents for Rat Factor VIII ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Factor VIII

Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).

Alternate Names

AHF, Antihemophilic factor, coagulation factor VIII, procoagulant component, coagulation factor VIIIc, DXS1253E, F8Ccoagulation factor VIII, factor VIII F8B, FVIII, HEMAF8B, Procoagulant component

Gene Symbol

F8

Additional Factor VIII Products

Product Documents for Rat Factor VIII ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Factor VIII ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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