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Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82512

Novus Biologicals, part of Bio-Techne
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NBP2-82512

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Fatty Acid Synthase/FASN in samples. No Significant cross-reactivity or interference between Human Fatty Acid Synthase/FASN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.66

Inter-Assay Precision (Precision between assays) CV% < 4.8

Recovery for Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

Recovery

86-100%

Linearity

Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

Scientific Data Images for Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

ELISA: Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) [NBP2-82512] -

ELISA: Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) [NBP2-82512] - Standard Curve Reference

Kit Contents for Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Fatty Acid Synthase/FASN

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Alternate Names

FASN, OA-519, SDR27X1

Gene Symbol

FASN

Additional Fatty Acid Synthase/FASN Products

Product Documents for Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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