Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00384

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Fc gamma RIIIB/CD16b ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Fc gamma RIIIB/CD16b in samples. No significant cross-reactivity or interference between Human Fc gamma RIIIB/CD16b and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.37%

Inter-Assay Precision (Precision between assays) 4.71%

Recovery for Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

Recovery

85 - 104%

Linearity

Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

Scientific Data Images for Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

ELISA: Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric) [NBP3-00384] -

ELISA: Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric) [NBP3-00384] - Standard Curve Reference

Kit Contents for Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Fc gamma RIIIB/CD16b

CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Long Name

Fc gamma Receptor III

Alternate Names

CD16b, FCG3, FCGR3B, FcgRIIIB, FcR-10, FCRIIIB, IGFR3

Gene Symbol

FCGR3B

Additional Fc gamma RIIIB/CD16b Products

Product Documents for Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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