Mouse GATA-1 ELISA Kit (Colorimetric)

Name: Mouse GATA-1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-00432

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00432

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse GATA-1 in samples. No significant cross-reactivity or interference between Mouse GATA-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.63%

Inter-Assay Precision (Precision between assays) 4.83%

Recovery for Mouse GATA-1 ELISA Kit (Colorimetric)

Recovery

85 - 107%

Linearity

Scientific Data Images for Mouse GATA-1 ELISA Kit (Colorimetric)

ELISA: Mouse GATA-1 ELISA Kit (Colorimetric) [NBP3-00432] -

ELISA: Mouse GATA-1 ELISA Kit (Colorimetric) [NBP3-00432] - Standard Curve Reference

Kit Contents for Mouse GATA-1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GATA-1

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Alternate Names

GATA1

Gene Symbol