Human Gelsolin/GSN ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82209

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

3.75 ng/mL

Assay Range

6.25 - 400 ng/mL

View all Gelsolin/GSN ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Gelsolin/GSN in samples. No Significant cross-reactivity or interference between Human Gelsolin/GSN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 6

Inter-Assay Precision (Precision between assays) %CV < 6

Recovery for Human Gelsolin/GSN ELISA Kit (Colorimetric)

Recovery

average = 102% for serum; average = 90% for EDTA plasma; average = 95% for cell culture media

Linearity

Human Gelsolin/GSN ELISA Kit (Colorimetric)

Scientific Data Images for Human Gelsolin/GSN ELISA Kit (Colorimetric)

Human Gelsolin/GSN ELISA Kit (Colorimetric)

ELISA: Human Gelsolin/GSN ELISA Kit (Colorimetric) [NBP2-82209] -

ELISA: Human Gelsolin/GSN ELISA Kit (Colorimetric) [NBP2-82209] - Standard Curve Reference

Kit Contents for Human Gelsolin/GSN ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Human Gelsolin/GSN ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Gelsolin/GSN ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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