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Human Ghrelin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66709

Novus Biologicals, part of Bio-Techne
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NBP2-66709

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes the acylated version of Human GHRL in samples. No significant cross-reactivity or interference between Human GHRL and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.78%

Inter-Assay Precision (Precision between assays) CV% < 5.08%

Recovery for Human Ghrelin ELISA Kit (Colorimetric)

Recovery

83-100%

Linearity

Human Ghrelin ELISA Kit (Colorimetric)

Scientific Data Images for Human Ghrelin ELISA Kit (Colorimetric)

Human Ghrelin ELISA Kit (Colorimetric)

ELISA: Human Ghrelin ELISA Kit (Colorimetric) [NBP2-66709] -

ELISA: Human Ghrelin ELISA Kit (Colorimetric) [NBP2-66709] - Standard Curve Reference

Kit Contents for Human Ghrelin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Ghrelin

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Alternate Names

GHRL, Motilin-related Peptide, Protein M46

Gene Symbol

GHRL

Additional Ghrelin Products

Product Documents for Human Ghrelin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Ghrelin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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