Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82203

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Glucocorticoid Receptor alpha in samples. No Significant cross-reactivity or interference between Human Glucocorticoid Receptor alpha and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5

Inter-Assay Precision (Precision between assays) %CV < 6

Recovery for Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

Recovery

average = 92% for serum; average = 91% for EDTA plasma; average = 95% for cell culture media

Linearity

Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

Scientific Data Images for Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

ELISA: Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric) [NBP2-82203] -

ELISA: Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric) [NBP2-82203] - Standard Curve Reference

Kit Contents for Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glucocorticoid Receptor alpha

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the Glucocorticoid Receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform translocates from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR alpha has a greater affinity for GR response elements (GREs) than GR beta only when in the ligand bound state.

Alternate Names

GCR, glucocorticoid receptor, GRGCCR, GRLNuclear receptor subfamily 3 group C member 1, nuclear receptor subfamily 3, group C, member 1, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Gene Symbol

NR3C1

Additional Glucocorticoid Receptor alpha Products

Product Documents for Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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