Mouse Glut2 ELISA Kit (Colorimetric)

Name: Mouse Glut2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-82169

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82169

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse GLUT2 in samples. No Significant cross-reactivity or interference between Mouse GLUT2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5%

Inter-Assay Precision (Precision between assays) CV% < 6%

Recovery for Mouse Glut2 ELISA Kit (Colorimetric)

Recovery

average = 91% for serum; average = 101% for EDTA plasma; average = 100% for cell culture media

Linearity

Scientific Data Images for Mouse Glut2 ELISA Kit (Colorimetric)

ELISA: Mouse Glut2 ELISA Kit (Colorimetric) [NBP2-82169] -

ELISA: Mouse Glut2 ELISA Kit (Colorimetric) [NBP2-82169] - Standard Curve Reference

Kit Contents for Mouse Glut2 ELISA Kit (Colorimetric)

Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glut2

Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as Glut 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. The majority of differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testis barriers.

Long Name

Glucose Transporter Type 2

Alternate Names

SLC2A2

Gene Symbol