Rat Glypican 3 ELISA Kit (Colorimetric)

Name: Rat Glypican 3 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-82186

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82186

Submit a review

Product Datasheet / COA / SDS / Protocols & Manuals

100% Guarantee

Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Glypican 3 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Glypican 3 in samples. No Significant cross-reactivity or interference between Rat Glypican 3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Rat Glypican 3 ELISA Kit (Colorimetric)

Recovery

average = 102% for serum; average = 102% for EDTA plasma; average = 97% for cell culture media

Linearity

Scientific Data Images for Rat Glypican 3 ELISA Kit (Colorimetric)

ELISA: Rat Glypican 3 ELISA Kit (Colorimetric) [NBP2-82186] -

ELISA: Rat Glypican 3 ELISA Kit (Colorimetric) [NBP2-82186] - Standard Curve Reference

Kit Contents for Rat Glypican 3 ELISA Kit (Colorimetric)

Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glypican 3

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Alternate Names

GPC3

Gene Symbol