Human GM-CSF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91277

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid (50 uL)

Sensitivity

2.9 pg/mL

Assay Range

7.8 - 500 pg/mL

View all GM-CSF ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human GM-CSF. The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a human GM-CSF positive serum. There was no crossreactivity detected.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 6.00

Inter-Assay Precision (Precision between assays) %CV < 4.40

Recovery for Human GM-CSF ELISA Kit (Colorimetric)

Recovery

85%

Scientific Data Images for Human GM-CSF ELISA Kit (Colorimetric)

ELISA: Human GM-CSF ELISA Kit (Colorimetric) [NBP1-91277]

ELISA: Human GM-CSF ELISA Kit (Colorimetric) [NBP1-91277]

ELISA: Human GM-CSF ELISA Kit (Colorimetric) [NBP1-91277] - Representative standard curve.

Kit Contents for Human GM-CSF ELISA Kit (Colorimetric)

  • Adhesive Plate Covers
  • Assay Buffer concentrate
  • Chromogen
  • HRP-conjugated Detection Antibody
  • Pre-coated 96 well plate
  • Sample Diluent
  • Standard
  • Stop Solution
  • Wash Buffer

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GM-CSF

Granulocyte - macrophage colony - stimulating factor (GM-CSF) is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes GM-CSF can be produced by a wide variety of tissue types, including fibroblasts, endothelial cells, T-cells, macrophages, mesothelial cells, epithelial cells and many types of tumor cells. In most of these tissues, inflammatory mediators, such as interleukin-1, interleukin-6, tumor necrosis factor or endotoxin, are inducers of GM-CSF gene expression, which occurs at least partly by post-transcriptional modification of the GM-CSF mRNA. The biological effects of GM-CSF are mediated through binding to cell surface receptors, which appear to be widely expressed by hematopoietic cells and also by some non-hematopoietic cells, such as endothelial cells. At least two different functional classes of GM-CSF receptor have been identified. The neutrophil GM-CSF receptor exclusively binds GM-CSF, while interleukin-3 competes for binding of GM-CSF to a second class of receptors detected on some leukemic cell lines. Monitoring of GM-CSF has been found relevant in human prostate cancer, poorly healing wounds, thyroid carcinoma, severe mucositis, fungal infections, AIDS, bone marrow transplantation, renal cell carcinoma and prostate cancer, acute lymphoblastic leukemia pulmonary inflammation, hematological malignancies, infection, lung cancer.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Gene Symbol

CSF2

Additional GM-CSF Products

Product Documents for Human GM-CSF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human GM-CSF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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