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Rat GRK2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66688

Novus Biologicals, part of Bio-Techne
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NBP2-66688

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat GRK2 in samples. No significant cross-reactivity or interference between Rat GRK2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.37%

Inter-Assay Precision (Precision between assays) CV% < 5.87%

Recovery for Rat GRK2 ELISA Kit (Colorimetric)

Recovery

85-103%

Linearity

Rat GRK2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat GRK2 ELISA Kit (Colorimetric)

Rat GRK2 ELISA Kit (Colorimetric)

ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] -

ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] - Standard Curve Reference

Kit Contents for Rat GRK2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GRK2

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Long Name

G protein-coupled Receptor Kinase 2

Alternate Names

ADRBK1, BARK1, Beta-ARK1

Gene Symbol

GRK2

Additional GRK2 Products

Product Documents for Rat GRK2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat GRK2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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