Human Hemoglobin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA2131

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Plasma, Serum (100 uL)

Sensitivity

1.378

Assay Range

6.25 to 200 ng/mL

View all Hemoglobin ELISA Kits »

Product Specifications

Assay Type

Two-site enzyme-linked immunoassay

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Hemoglobin (Human) ELISA Kit is a two-site enzyme linked immunoassay for the measurement of human Hemoglobin.

Kit Contents for Human Hemoglobin ELISA Kit (Colorimetric)

  • Anti-Human Hemoglobin ELISA Micro Plate: Twelve removable eight (8) well strips in well holder frame. Each well is coated with affinity purified anti-Human Hemoglobin
  • Chromogen-Substrate Solution: One vial containing 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  • Diluent Concentrate (Running buffer): One bottle containing 5X concentrated diluent running buffer.
  • Enzyme-Antibody Conjugate 100X: One vial containing affinity purified anti-Human Hemoglobin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  • Human Hemoglobin Calibrator: One vial containing a lyophilized Human Hemoglobin Calibrator.
  • Stop Solution: One vial containing 0.3 M sulfuric acid. WARNING: Avoid contact with skin
  • Wash Solution Concentrate: One bottle containing 20X concentrated wash solution.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Hemoglobin

Hemoglobin is a tetrameric protein that serves as the oxygen transporter in red blood cells. Two alpha chain and two beta chains comprise the adult form of the molecule, with each subunit containing a heme group, or oxygen binding site. Sickle cell anemia is caused by a single amino acid change in the beta chain, which alters the solubility of the deoxygenated form of the molecule and causes the formation of long fibers that distort the red blood cell into the characteristic sickle shape. Thalassemias, another set of genetic diseases associated with hemoglobin, result from absence or deficiency in one of the hemoglobin chains.

Alternate Names

alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1

Additional Hemoglobin Products

Product Documents for Human Hemoglobin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Hemoglobin ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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