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Porcine Hemoglobin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42695

Novus Biologicals, part of Bio-Techne
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NBP3-42695

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Erythrocyte lysates and other biological fluids (50 uL)

Sensitivity

1.41ug/mL

Assay Range

3.70 - 300ug/mL

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Description

Assay time: 2 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Porcine Hemoglobin ELISA Kit (Colorimetric)

Recovery

82 - 105%

Kit Contents for Porcine Hemoglobin ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Hemoglobin

Hemoglobin is a tetrameric protein that serves as the oxygen transporter in red blood cells. Two alpha chain and two beta chains comprise the adult form of the molecule, with each subunit containing a heme group, or oxygen binding site. Sickle cell anemia is caused by a single amino acid change in the beta chain, which alters the solubility of the deoxygenated form of the molecule and causes the formation of long fibers that distort the red blood cell into the characteristic sickle shape. Thalassemias, another set of genetic diseases associated with hemoglobin, result from absence or deficiency in one of the hemoglobin chains.

Alternate Names

alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1

Additional Hemoglobin Products

Product Documents for Porcine Hemoglobin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Porcine Hemoglobin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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