HIV-1 Gag p24 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DHP240B
Validated for Cell Culture Supernates Only
Key Product Details
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL)
Sensitivity
3.35 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates)
Product Summary for HIV-1 Gag p24 Quantikine ELISA Kit
The Quantikine™ HIV-1 Gag p24 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure HIV-1 Gag p24 in cell culture supernates.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Viral
Specificity
This assay recognizes HIV-1 Gag p24.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 45.7 | 135 | 280 | 43.0 | 132 | 285 |
| Standard Deviation | 1.41 | 4.86 | 8.10 | 1.93 | 4.61 | 11.2 |
| CV% | 3.1 | 3.6 | 2.9 | 4.5 | 3.5 | 3.9 |
Recovery for HIV-1 Gag p24 Quantikine ELISA Kit
The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 93-105 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of HIV-1 Gag p24 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for HIV-1 Gag p24 Quantikine ELISA Kit
HIV-1 Gag p24 Quantikine ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HIV-1 Gag p24
Long Name
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Alternate Names
CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)
Entrez Gene IDs
155030 (Viral)
Gene Symbol
GAG
Additional HIV-1 Gag p24 Products
Product Documents for HIV-1 Gag p24 Quantikine ELISA Kit
Product Specific Notices for HIV-1 Gag p24 Quantikine ELISA Kit
For research use only
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