Mouse HSP20/HSPB6 ELISA Kit (Colorimetric)

Name: Mouse HSP20/HSPB6 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-76437

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76437

Submit a review

Product Datasheet / COA / SDS / Protocols & Manuals

100% Guarantee

Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all HSP20/HSPB6 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse HSP-20 in samples. No significant cross-reactivity or interference between Mouse HSP-20 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.14%

Inter-Assay Precision (Precision between assays) CV% < 5.49%

Recovery for Mouse HSP20/HSPB6 ELISA Kit (Colorimetric)

Recovery

86-107%

Linearity

Scientific Data Images for Mouse HSP20/HSPB6 ELISA Kit (Colorimetric)

ELISA: Mouse HSP20/HSPB6 ELISA Kit (Colorimetric) [NBP2-76437] -

ELISA: Mouse HSP20/HSPB6 ELISA Kit (Colorimetric) [NBP2-76437] - Standard Curve Reference

Kit Contents for Mouse HSP20/HSPB6 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HSP20/HSPB6

Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction.

Long Name

Heat Shock Protein 20

Alternate Names

HSPB6

Gene Symbol