Human HSP27 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0981

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Samples, Plasma, Serum, Milk, Tissue Extract (50 uL)

Sensitivity

0.16 ng/mL

Assay Range

0.313 to 80 ng/mL

View all HSP27 ELISA Kits »

Product Specifications

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Quality control test: Standard curve. HSP27 (Human) ELISA Kit is designed for detection of human HSP27 in plasma, serum, milk, tissue extract, and cell culture samples.

Scientific Data Images for Human HSP27 ELISA Kit (Colorimetric)

ELISA: Human HSP27 ELISA Kit (Colorimetric) [KA0981]

ELISA: Human HSP27 ELISA Kit (Colorimetric) [KA0981]

ELISA: Human HSP27 ELISA Kit (Colorimetric) [KA0981] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human HSP27 ELISA Kit (Colorimetric)

  • Biotinylated Human HSP27 Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against HSP27.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine
  • EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  • Human HSP27 Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human HSP27
  • Human HSP27 Standard: Human HSP27 in a buffered protein base (lyophilized).
  • Sealing Tapes: pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydroxychloric acid to stop the chromogen substrate reaction
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for Human HSP27 ELISA Kit (Colorimetric)

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Certificate of Analysis

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Product Specific Notices for Human HSP27 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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