Human ADAMTS13 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DADT130
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
0.26 ng/mL
Assay Range
0.781-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human ADAMTS13 Quantikine ELISA Kit
The Quantikine Human ADAMTS13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ADAMTS13 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human ADAMTS13 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ADAMTS13 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ADAMTS13.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human ADAMTS13. This kit also recognizes a truncated version of recombinant human ADAMTS13 (amino acids Gln34-Trp688; Accession # NP_620594).
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 4.78 | 13.8 | 29.7 | 4.51 | 14.2 | 30.2 |
| Standard Deviation | 0.177 | 0.364 | 0.685 | 0.256 | 0.722 | 1.08 |
| CV% | 3.7 | 2.6 | 2.3 | 5.7 | 5.1 | 3.6 |
Recovery for Human ADAMTS13 Quantikine ELISA Kit
The recovery of ADAMTS13 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 100 | 91-111 |
| Citrate Plasma (n=4) | 97 | 87-114 |
| EDTA Plasma (n=4) | 97 | 89-109 |
| Heparin Plasma (n=4) | 97 | 85-115 |
| Serum (n=4) | 97 | 85-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAMTS13 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human ADAMTS13 Quantikine ELISA Kit
Human ADAMTS13 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ADAMTS13
Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13
Alternate Names
TTP, vWF-CP
Gene Symbol
ADAMTS13
Additional ADAMTS13 Products
Product Documents for Human ADAMTS13 Quantikine ELISA Kit
Product Specific Notices for Human ADAMTS13 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.