Human beta 2-Microglobulin Parameter Assay Kit
R&D Systems, part of Bio-Techne | Catalog # KGE019
Key Product Details
Assay Length
1.5 hours
Sample Type & Volume Required Per Well
Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Urine (10 µL)
Sensitivity
0.132 µg/mL
Assay Range
0.15-9.6 µg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human beta 2-Microglobulin Parameter Assay Kit
The Parameter beta2-Microglobulin Immunoassay is a 1.5 hour forward sequential competitive enzyme immunoassay designed to measure beta2M in serum, plasma, and urine.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Human
Specificity
Measures Human beta2M levels in various samples.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three controls of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three controls of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (µg/mL) | 1.14 | 3.19 | 6.41 | 0.953 | 3.10 | 6.17 |
| Standard Deviation | 0.086 | 0.189 | 0.383 | 0.175 | 0.293 | 0.496 |
| CV% | 7.5 | 5.9 | 6.0 | 18.4 | 9.5 | 8.0 |
Recovery for Human beta 2-Microglobulin Parameter Assay Kit
The recovery of human beta2M spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 99 | 79-114 |
| Heparin Plasma (n=4) | 106 | 91-124 |
| Serum (n=4) | 101 | 85-122 |
| Urine (n=4) | 90 | 76-105 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human beta2M were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human beta 2-Microglobulin Parameter Assay Kit
Human beta2-Microglobulin Assay ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Gene Symbol
B2M
Additional beta 2-Microglobulin Products
Product Documents for Human beta 2-Microglobulin Parameter Assay Kit
Product Specific Notices for Human beta 2-Microglobulin Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.