Human C1qR1/CD93 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCD930
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (25 µL), Urine (50 µL)
Sensitivity
0.028 ng/mL
Assay Range
0.078-5 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human C1qR1/CD93 Quantikine ELISA Kit
The Quantikine Human C1q R1 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human C1q R1 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human C1q R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human C1q R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human C1q R1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human C1q R1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.49 | 1.58 | 3.41 | 0.48 | 1.51 | 3.19 |
| Standard Deviation | 0.01 | 0.05 | 0.11 | 0.03 | 0.09 | 0.15 |
| CV% | 2.0 | 3.2 | 3.2 | 6.3 | 6.0 | 4.7 |
Recovery for Human C1qR1/CD93 Quantikine ELISA Kit
The recovery of C1q R1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 99 | 92-108 |
| EDTA Plasma (n=4) | 104 | 94-118 |
| Heparin Plasma (n=4) | 102 | 91-115 |
| Saliva (n=4) | 104 | 94-112 |
| Serum (n=4) | 102 | 87-115 |
| Urine (n=4) | 97 | 93-102 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of C1q R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human C1qR1/CD93 Quantikine ELISA Kit
Human C1q R1/CD93 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C1qR1/CD93
Long Name
Complement Component C1q Receptor
Alternate Names
AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68
Gene Symbol
CD93
Additional C1qR1/CD93 Products
Product Documents for Human C1qR1/CD93 Quantikine ELISA Kit
Product Specific Notices for Human C1qR1/CD93 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.