Human CA125/MUC16 Quantikine ELISA Kit Best Seller

Name: Human CA125/MUC16 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DCA125
Rating: 5 (5 reviews)

R&D Systems, part of Bio-Techne | Catalog # DCA125

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (10 µL), Urine (100 µL), Human Milk (10 µL)

Sensitivity

0.099 U/mL

Assay Range

0.5-32 U/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

View all CA125/MUC16 ELISA Kits »

Product Summary for Human CA125/MUC16 Quantikine ELISA Kit

The Quantikine Human CA125/MUC16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA125 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human CA125 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA125 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA125.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human CA125

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	4.26	10.0	19.0	4.22	9.74	18.7
Standard Deviation	0.066	0.101	0.266	0.313	0.527	0.666
CV%	1.5	1.0	1.4	7.4	5.4	3.6

Recovery for Human CA125/MUC16 Quantikine ELISA Kit

The recovery of CA125 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	95-102
EDTA Plasma (n=4)	97	93-101
Heparin Plasma (n=4)	97	92-100
Serum (n=4)	97	90-101

Linearity

To assess the linearity of the assay, samples containing high concentrations of CA125 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human CA125/MUC16 Quantikine ELISA Kit

Human CA125/MUC16 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CA125/MUC16

The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Entrez Gene IDs

94025 (Human)

Gene Symbol