Human CCL11/Eotaxin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTX00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Citrate Plasma (50 µL)
Sensitivity
5 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Product Summary for Human CCL11/Eotaxin Quantikine ELISA Kit
The Quantikine Human Eotaxin Immunoassay is a 3.5 hour solid phase ELISA designed to measure human Eotaxin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Eotaxin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human Eotaxin accurately. Results obtained measuring natural human eotaxin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Eotaxin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 66.8 | 184 | 390 | 67.5 | 193 | 396 |
| Standard Deviation | 2.0 | 8.4 | 23.5 | 7.8 | 18.2 | 40.2 |
| CV% | 3.0 | 4.6 | 6.0 | 11.6 | 9.4 | 10.2 |
Citrate Plasma, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 64.5 | 109 | 377 | 63.4 | 190 | 400 |
| Standard Deviation | 2.5 | 5.8 | 12.8 | 7.3 | 19.6 | 33.5 |
| CV% | 3.9 | 5.3 | 3.4 | 11.5 | 10.3 | 8.4 |
Recovery for Human CCL11/Eotaxin Quantikine ELISA Kit
The recovery of Eotaxin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 100 | 86-111 |
| Citrate Plasma (n=5) | 99 | 81-117 |
| EDTA Plasma (n=5) | 99 | 82-115 |
| Serum (n=5) | 95 | 83-109 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of Eotaxin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL11/Eotaxin Quantikine ELISA Kit
Human CCL11/Eotaxin ELISA Cell Culture Supernate Standard Curve
Human CCL11/Eotaxin ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL11/Eotaxin
Additional CCL11/Eotaxin Products
Product Documents for Human CCL11/Eotaxin Quantikine ELISA Kit
Product Specific Notices for Human CCL11/Eotaxin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.