Human CCL17/TARC ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DDN00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
7 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CCL17/TARC Quantikine ELISA Kit
The Quantikine Human TARC Immunoassay kit is a 3.5 hour solid phase ELISA designed to measure TARC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TARC and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TARC. Results obtained using natural human TARC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TARC.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human TARC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human TARC in this assay. No medical histories were available for the donors used in this
study.
*Two serum samples and one EDTA plasma sample measured substantially higher and are not included
in this range.
ND=Non-detectable
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Serume (n=66)* | 331 | 71-848 |
| EDTA plasma (n=34)* | 134 | 35-285 |
| Heparin plasma (n=35) | 111 | 33-226 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106
cells/mL)
were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were
cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates
were removed on days 1 and 5 and assayed for levels of human TARC.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | ND | 1880 |
| Stimulated | 1336 | 1696 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 88 | 504 | 1001 | 89 | 540 | 1033 |
| Standard Deviation | 4.1 | 21.7 | 27.2 | 8.3 | 38.9 | 71.7 |
| CV% | 4.7 | 4.3 | 2.7 | 9.3 | 7.2 | 6.9 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 87 | 600 | 1107 | 98 | 616 | 1178 |
| Standard Deviation | 6.2 | 13.7 | 29.9 | 8.9 | 50.7 | 88.8 |
| CV% | 7.1 | 2.3 | 2.7 | 9.1 | 8.2 | 7.5 |
Recovery for Human CCL17/TARC Quantikine ELISA Kit
The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 88-104 |
| EDTA Plasma (n=5) | 102 | 86-111 |
| Heparin Plasma (n=5) | 98 | 86-107 |
| Serum (n=5) | 105 | 100-114 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TARC were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL17/TARC Quantikine ELISA Kit
Human CCL17/TARC ELISA Calibrator Diluent RD5-5 Standard Curve
Human CCL17/TARC ELISA Calibrator Diluent RD6Q Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL17/TARC
Additional CCL17/TARC Products
Product Documents for Human CCL17/TARC Quantikine ELISA Kit
Product Specific Notices for Human CCL17/TARC Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.