Human CCL19/MIP-3 beta QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK0361
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
0.695 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CCL19/MIP-3 beta QuicKit ELISA
The Quantikine QuicKit Human CCL19/MIP-3 beta Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human MIP-3 beta levels in cell culture supernates, serum, and plasma. It contains recombinant human MIP-3 beta and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 beta showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural MIP-3 beta .
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MIP-3 beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 42.4 | 326 | 45.8 | 338 |
| Standard Deviation | 3.15 | 18.8 | 3.36 | 26.7 |
| CV% | 7.4 | 5.8 | 7.3 | 7.9 |
Recovery for Human CCL19/MIP-3 beta QuicKit ELISA
The recovery of human MIP-3 beta spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 112 | 106-117 |
| EDTA Plasma (n=2) | 99 | 91-107 |
| Heparin Plasma (n=2) | 98 | 93-102 |
| Serum (n=2) | 98 | 94-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human MIP-3 beta were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human CCL19/MIP-3 beta QuicKit ELISA
Human CCL19/MIP-3 beta Quantikine QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL19/MIP-3 beta
Alternate Names
ELC, MIP-3 beta, MIP3 beta
Gene Symbol
CCL19
Additional CCL19/MIP-3 beta Products
Product Documents for Human CCL19/MIP-3 beta QuicKit ELISA
Product Specific Notices for Human CCL19/MIP-3 beta QuicKit ELISA
For research use only
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