Human CCL20/MIP-3 alpha Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DM3A00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.87 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CCL20/MIP-3 alpha Quantikine ELISA Kit
The Quantikine Human MIP-3 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure MIP-3 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-3 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-3 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MIP-3 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 66.2 | 180 | 372 | 68.7 | 186 | 357 |
| Standard Deviation | 1.84 | 3.83 | 15.1 | 4.51 | 13.0 | 28.8 |
| CV% | 2.8 | 2.1 | 4.1 | 6.6 | 6.9 | 8.1 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 55.9 | 161 | 323 | 72.5 | 200 | 386 |
| Standard Deviation | 1.62 | 4.28 | 9.10 | 6.05 | 17.9 | 35.3 |
| CV% | 2.9 | 2.7 | 2.8 | 8.3 | 8.9 | 9.1 |
Recovery for Human CCL20/MIP-3 alpha Quantikine ELISA Kit
The recovery of MIP-3 alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 101 | 88-115 |
| EDTA Plasma (n=5) | 104 | 97-115 |
| Heparin Plasma (n=5) | 104 | 98-112 |
| Serum (n=5) | 103 | 94-112 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of MIP-3 alpha in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL20/MIP-3 alpha Quantikine ELISA Kit
Human CCL20/MIP-3 alpha ELISA Calibrator Diluent RD6U Standard Curve
Human CCL20/MIP-3 alpha ELISA Calibrator Diluent RD6-21 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL20/MIP-3 alpha
Alternate Names
exodus-1, LARC, MIP-3 alpha, MIP3 alpha
Gene Symbol
CCL20
Additional CCL20/MIP-3 alpha Products
Product Documents for Human CCL20/MIP-3 alpha Quantikine ELISA Kit
Product Specific Notices for Human CCL20/MIP-3 alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.