Human CCL4/MIP-1 beta Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DMB00
Key Product Details
Assay Length
3.0 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (150 µL), EDTA Plasma (150 µL), Heparin Plasma (150 µL), Citrate Plasma (150 µL)
Sensitivity
4 pg/mL (Cell Culture Supernates)
11 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
11 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates)
31.2-2000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
31.2-2000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human CCL4/MIP-1 beta Quantikine ELISA Kit
The Quantikine Human MIP-1 beta Immunoassay is a 3.0 or 4.5 hour solid phase ELISA designed to measure MIP-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 beta (Act-2 variant) and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant human MIP-1 beta. Results obtained using natural human MIP-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-1 beta.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MIP-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 23.3 | 98.8 | 428 | 31.4 | 118 | 476 |
| Standard Deviation | 1.1 | 3.8 | 17.3 | 3.7 | 9.9 | 28.0 |
| CV% | 4.7 | 3.8 | 4.0 | 11.8 | 8.4 | 5.9 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 51.3 | 208 | 932 | 58.8 | 220 | 949 |
| Standard Deviation | 4.6 | 7.5 | 30.2 | 5.7 | 15.6 | 70.5 |
| CV% | 9.0 | 3.6 | 3.2 | 9.7 | 7.1 | 7.4 |
Recovery for Human CCL4/MIP-1 beta Quantikine ELISA Kit
The recovery of MIP-1 beta spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 107 | 81-117 |
| Citrate Plasma (n=5) | 99 | 87-116 |
| EDTA Plasma (n=5) | 100 | 84-112 |
| Heparin Plasma (n=5) | 99 | 90-112 |
| Serum (n=5) | 99 | 88-113 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of MIP-1 beta in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL4/MIP-1 beta Quantikine ELISA Kit
Human CCL4/MIP-1 beta ELISA Cell Culture Supernate Standard Curve
Human CCL4/MIP-1 beta ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL4/MIP-1 beta
Alternate Names
Exodus-3, MIP-1 beta, MIP1 beta
Entrez Gene IDs
Gene Symbol
CCL4
Additional CCL4/MIP-1 beta Products
Product Documents for Human CCL4/MIP-1 beta Quantikine ELISA Kit
Product Specific Notices for Human CCL4/MIP-1 beta Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.