Human CD23/Fc epsilon RII Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCD230
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (30 µL), EDTA Plasma (30 µL), Heparin Plasma (30 µL), Urine (30 µL)
Sensitivity
5.1 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human CD23/Fc epsilon RII Quantikine ELISA Kit
The Quantikine Human CD23 immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD23 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CD23 (extracellular domain) and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD23 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring soluble CD23.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human CD23. This assay recognizes the 25 kDa fragment (aa 150-321) and the full length extracellular domain (aa 48-321) of human CD23/Fc epsilon RII.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 158 | 283 | 576 | 151 | 274 | 533 |
| Standard Deviation | 13.5 | 17.8 | 40.8 | 13.7 | 22.4 | 40.2 |
| CV% | 8.5 | 6.3 | 7.1 | 9.1 | 8.2 | 7.5 |
Recovery for Human CD23/Fc epsilon RII Quantikine ELISA Kit
The recovery of CD23 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 94-114 |
| EDTA Plasma (n=4) | 99 | 89-105 |
| Heparin Plasma (n=4) | 98 | 90-112 |
| Serum (n=4) | 94 | 85-108 |
| Urine (n=4) | 100 | 85-113 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of CD23 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CD23/Fc epsilon RII Quantikine ELISA Kit
Human CD23/Fc epsilon RII ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD23/Fc epsilon RII
Long Name
Fc epsilon Receptor II
Alternate Names
CD23, CLEC4J, Fc epsilon RII, FCER2, Fcer2a, FceRII, IGEBF, Ly-42
Gene Symbol
FCER2
Additional CD23/Fc epsilon RII Products
Product Documents for Human CD23/Fc epsilon RII Quantikine ELISA Kit
Product Specific Notices for Human CD23/Fc epsilon RII Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.