Human CD45 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY1430-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
78.1-5000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human CD45 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human CD45 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CD45. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human CD45 DuoSet ELISA
Human CD45 DuoSet ELISA Standard Curve
Kit Contents for Human CD45 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008B) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (R&D Systems,
Catalog #DY999B)
Stop Solution:2N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990)
Plate Sealers:ELISA Plate Sealers (Catalog #DY992)
*For the Reagent Diluent and Blocking Buffer arerecommended for a specific DuoSet ELISA Development Kit,see the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD45
CD45 is a transmembrane protein tyrosine phosphatase. It is expressed at high levels on the cell surface of all nucleated hematopoietic cells and their precursors. Alternative splicing of CD45 mRNA results in the expression of several distinct isoforms that are differentially expressed in cells of different lineages and activation states.
Long Name
Cluster of Differentiation 45
Alternate Names
CD45, LCA, PTPRC, T200 Glycoprotein
Gene Symbol
PTPRC
Additional CD45 Products
Product Documents for Human CD45 DuoSet ELISA
Product Specific Notices for Human CD45 DuoSet ELISA
For research use only
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