Human Chymase/CMA1 DuoSet ELISA

Name: Human Chymase/CMA1 DuoSet ELISA
Brand: R&D Systems
SKU: DY4099-05

R&D Systems, part of Bio-Techne | Catalog # DY4099-05

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

93.8-6000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Chymase/CMA1 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all Chymase/CMA1 ELISA Kits »

Product Summary for Human Chymase/CMA1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Chymase. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as tissue lysate, serum, and plasma should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Chymase/CMA1 DuoSet ELISA

Human Chymase ELISA Standard Curve

Kit Contents for Human Chymase/CMA1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chymase/CMA1

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells. They are synthesized as inactive precursors containing a 2-residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for enzymatic activity. Human chymase encoded by the CMA1 gene is known to be involved in hypertension and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines.

Alternate Names

CMA1, CYH, MCP3P, Mcpt5, MCT1

Entrez Gene IDs

1215 (Human); 17228 (Mouse); 25627 (Rat)

Gene Symbol

CMA1

Additional Chymase/CMA1 Products

Product Documents for Human Chymase/CMA1 DuoSet ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Chymase/CMA1 DuoSet ELISA

For research use only

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