Human CNTF Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DNT00
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
8 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human CNTF Quantikine ELISA Kit
The Quantikine Human CNTF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure recombinant human CNTF in cell culture supernates, serum, and plasma. It contains recombinant human CNTF and has been shown to accurately quantitate the recombinant factor.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Recombinant human CNTF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 110 | 461 | 1295 | 128 | 399 | 821 |
| Standard Deviation | 5.0 | 10.2 | 31.4 | 10.1 | 26.8 | 52.6 |
| CV% | 4.5 | 2.2 | 2.4 | 7.9 | 6.7 | 6.4 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 103 | 406 | 1201 | 206 | 671 | 1388 |
| Standard Deviation | 4.4 | 12.0 | 28.2 | 19.6 | 47.8 | 93.9 |
| CV% | 4.3 | 3.0 | 2.3 | 9.5 | 7.1 | 6.8 |
Recovery for Human CNTF Quantikine ELISA Kit
The recovery of CNTF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 99 | 92-115 |
| Citrate Plasma (n=5) | 87 | 71-125 |
| EDTA Plasma (n=5) | 90 | 78-97 |
| Heparin Plasma (n=5) | 92 | 82-98 |
| Serum (n=5) | 95 | 85-101 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of CNTF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CNTF Quantikine ELISA Kit
Human CNTF ELISA Cell Culture Supernate Standard Curve
Human CNTF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CNTF
Long Name
Ciliary Neurotrophic Factor
Alternate Names
ciliary neurotrophic factor, HCNTF
Gene Symbol
CNTF
Additional CNTF Products
Product Documents for Human CNTF Quantikine ELISA Kit
Product Specific Notices for Human CNTF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.