Human CX3CL1/Fractalkine Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCX310
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (50 µL), Urine (50 µL), Human Milk (100 µL)
Sensitivity
0.072 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human CX3CL1/Fractalkine Quantikine ELISA Kit
The Quantikine Human Fractalkine immunoassay is a 4.5 hour solid phase ELISA designed to measure Fractalkine in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Fractalkine and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Fractalkine showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fractalkine.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Fractalkine. This assay detects all forms of human Fractalkine containing the chemokine domain.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.18 | 3.33 | 6.43 | 1.19 | 3.53 | 6.85 |
| Standard Deviation | 0.03 | 0.11 | 0.24 | 0.07 | 0.15 | 0.31 |
| CV% | 2.5 | 3.4 | 3.7 | 6.1 | 4.3 | 4.5 |
EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.24 | 3.80 | 7.12 | 1.23 | 3.59 | 6.88 |
| Standard Deviation | 0.04 | 0.08 | 0.17 | 0.11 | 0.20 | 0.37 |
| CV% | 3.2 | 2.1 | 2.4 | 8.9 | 5.6 | 5.4 |
Recovery for Human CX3CL1/Fractalkine Quantikine ELISA Kit
The recovery of Fractalkine spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 93-102 |
| EDTA Plasma (n=4) | 101 | 88-113 |
| Heparin Plasma (n=4) | 97 | 85-108 |
| Serum (n=4) | 99 | 88-111 |
| Urine (n=4) | 96 | 86-109 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of Fractalkine were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CX3CL1/Fractalkine Quantikine ELISA Kit
Human CX3CL1/Fractalkine ELISA Cell Culture Supernate/Saliva Standard Curve
Human CX3CL1/Fractalkine ELISA Serum/Plasma/Urine/Human Milk Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CX3CL1/Fractalkine
Alternate Names
FKN, Fractalkine, Neurotactin
Gene Symbol
CX3CL1
Additional CX3CL1/Fractalkine Products
Product Documents for Human CX3CL1/Fractalkine Quantikine ELISA Kit
Product Specific Notices for Human CX3CL1/Fractalkine Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.