Human CXCL10/IP-10 Quantikine QuicKit ELISA Best Seller
R&D Systems, part of Bio-Techne | Catalog # QK266
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
4.1 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CXCL10/IP-10 Quantikine QuicKit ELISA
The Quantikine® QuicKit™ Human CXCL10/IP-10 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IP-10 levels in cell culture supernates, serum, and plasma. It contains E.
coli-expressed recombinant human IP-10 and antibodies raised against the recombinant protein. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human IP-10.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IP-10.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human IP-10 in this assay. No medical histories were available for the donors used in this
study.
Cell Culture Supernates:
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation |
| Serum (n=10) | 85.6 | 49.8-250 | 62.0 |
| EDTA plasma (n=10) | 99.4 | 60.4-205 | 51.8 |
| Heparin plasma (n=10) | 137 | 83.9-281 | 75.0 |
Cell Culture Supernates:
Human peripheral blood cells (PBMCs) (1 x 106
cells/mL) were cultured in RPMI supplemented
with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL
streptomycin sulfate. Cells were left unstimulated or stimulated with 10 μg/mL PHA for
5 days. Aliquots of the cell culture supernates were removed, assayed for levels of human IP-10,
and measured 12,555 pg/mL and 6570 pg/mL, respectively.
PBMCs were cultured in the medium as described above. Cells were left unstimulated or
stimulated with 1 μg/mL LPS and 40 ng/mL of recombinant human IFN-gamma for 24 hours. Aliquots
of the cell culture supernates were removed, assayed for levels of human IP-10, and measured
174 pg/mL and 958 pg/mL, respectively.
THP-1 human acute monocyte leukemia cells were left in RPMI supplemented with
10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin,
and 100 μg/mL streptomycin sulfate. Cells were left unstimulated or stimulated with
1 μg/mL of recombinant human IFN-gamma for 8 hours and then 1.0 μg/mL LPS was added. Cells
were incubated for an additional 18 hours. Aliquots of the cell culture supernates were
removed, assayed for levels of human IP-10, and measured 1172 pg/mL and 270,600 pg/mL,
respectively.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 96.7 | 521 | 95.3 | 515 |
| Standard Deviation | 1.12 | 10.6 | 10.8 | 42.1 |
| CV% | 1.2 | 2.0 | 11.3 | 8.2 |
Recovery for Human CXCL10/IP-10 Quantikine QuicKit ELISA
The recovery of human IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 119 | 110-129 |
| EDTA Plasma (n=2) | 86 | 78-95 |
| Heparin Plasma (n=2) | 104 | 92-118 |
| Serum (n=2) | 71 | 67-76 |
Linearity
Scientific Data Images for Human CXCL10/IP-10 Quantikine QuicKit ELISA
Human IP-10 ELISA Standard Curve
Human IP-10 QuicKit Spiked Recovery Competitor Comparison
IP-10 is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 95% compared to 49% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
Human IP-10 QuicKit Spiked Linearity Competitor Comparison
IP-10 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-124% compared to 112%-183% for the top competitor.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL10/IP-10/CRG-2
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Human CXCL10/IP-10 Quantikine QuicKit ELISA
Product Specific Notices for Human CXCL10/IP-10 Quantikine QuicKit ELISA
For research use only
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