Human CXCL5/ENA-78 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DX000
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (50 µL), Platelet-poor Heparin Plasma (50 µL), Citrate Plasma (50 µL)
Sensitivity
15 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
Product Summary for Human CXCL5/ENA-78 Quantikine ELISA Kit
The Quantikine Human ENA-78 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human ENA-78 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human ENA-78 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human ENA-78 accurately. Results obtained using natural human ENA-78 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ENA-78.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human ENA-78
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 112 | 251 | 1096 | 129 | 278 | 1085 |
| Standard Deviation | 6.0 | 14.6 | 44.9 | 12.6 | 26.8 | 96.5 |
| CV% | 5.4 | 5.8 | 4.1 | 9.8 | 9.6 | 8.9 |
Citrate Plasma, EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 113 | 244 | 995 | 109 | 240 | 989 |
| Standard Deviation | 9.4 | 12.9 | 37.8 | 10.1 | 17.8 | 65.8 |
| CV% | 8.3 | 5.3 | 3.8 | 9.3 | 7.4 | 6.7 |
Recovery for Human CXCL5/ENA-78 Quantikine ELISA Kit
The recovery of ENA-78 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 101 | 95-107 |
| Citrate Plasma (n=5) | 100 | 94-106 |
| EDTA Plasma (n=5) | 98 | 92-104 |
| Heparin Plasma (n=5) | 98 | 93-103 |
| Serum (n=5) | 101 | 93-109 |
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of ENA-78 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL5/ENA-78 Quantikine ELISA Kit
Human CXCL5/ENA-78 ELISA Cell Culture Supernate Standard Curve
Human CXCL5/ENA-78 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL5/ENA-78
Additional CXCL5/ENA-78 Products
Product Documents for Human CXCL5/ENA-78 Quantikine ELISA Kit
Product Specific Notices for Human CXCL5/ENA-78 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.