Human CXCL9/MIG Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DCX900
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
11.3 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CXCL9/MIG Quantikine ELISA Kit
The Quantikine Human MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MIG in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MIG.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MIG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human MIG in this assay. No medical histories were available for the donors used in this
study.
ND=Non-detectable
ND=Non-detectable
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum (n=40) | 64.4 | 65 | ND-199 |
| EDTA plasma (n=23) | 56.7 | 62 | ND-179 |
| Heparin plasma (n=25) | 62.0 | 68 | ND-209 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106
cells/mL) were
cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol, 2
mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured
unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were
removed and assayed for levels of natural human MIG.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | ND | 445 |
| Stimulated | 77.5 | 12,290 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 235 | 700 | 1358 | 242 | 707 | 1391 |
| Standard Deviation | 9.5 | 25.1 | 24.3 | 20.8 | 50.9 | 83.6 |
| CV% | 4.0 | 3.6 | 1.8 | 8.6 | 7.2 | 6.0 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 253 | 817 | 1603 | 245 | 723 | 1427 |
| Standard Deviation | 9.9 | 26.6 | 49.6 | 12.8 | 59.4 | 88.1 |
| CV% | 3.9 | 3.3 | 3.1 | 5.2 | 8.2 | 6.2 |
Recovery for Human CXCL9/MIG Quantikine ELISA Kit
The recovery of MIG spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 92 | 86-98 |
| EDTA Plasma (n=5) | 100 | 95-110 |
| Heparin Plasma (n=5) | 109 | 101-114 |
| Serum (n=5) | 108 | 101-113 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of MIG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL9/MIG Quantikine ELISA Kit
Human CXCL9/MIG ELISA Cell Culture Supernate Standard Curve
Human CXCL9/MIG ELISA Serum/Plasma Standard Curve
Human CXCL9/MIG Quantikine ELISA Kit
Serum ELISA results of three protein biomarkers in renal transplantation.We measured the protein concentration of ten genes by ELISA in independent serum samples of 19 AR patients and 20 patients with stable (STA) graft function after renal transplant. The protein concentrations of PECAM1 (A), CXCL9 (B), and CD44 (C) were higher in the AR serum samples, as shown in the notched boxplots. When the notches about two medians do not overlap, the medians are roughly significantly different at about a 95% confidence level [54] . The circles are outliers. P-values were calculated using the Mann-Whitney U test. One of the AR samples was inadvertently lost during the PECAM1 experiment and could not be recovered. (D) Areas under the Receiver Operating Characteristic (ROC) curves used to distinguish AR from STA were 0.811, 0.864, and 0.761 for PECAM1, CXCL9, and CD44, respectively. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pcbi.1000940), licensed under a CC-BY license. Not internally tested by R&D Systems.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL9/MIG
Additional CXCL9/MIG Products
Product Documents for Human CXCL9/MIG Quantikine ELISA Kit
Product Specific Notices for Human CXCL9/MIG Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.