Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit Best Seller

Name: Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DB7H10
Rating: 5 (4 reviews)

R&D Systems, part of Bio-Techne | Catalog # DB7H10

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Sample Values
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Cell Lysates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)

Sensitivity

4.52 pg/mL

Assay Range

25-1600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)

View all PD-L1/B7-H1 ELISA Kits »

Product Summary for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

The Quantikine^® Human/Cynomolgus Monkey B7-H1/PD-L1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human/cynomolgus monkey B7-H1 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains HEK293-expressed recombinant human B7-H1/PD-L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/cynomolgus monkey B7-H1/PD-L1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human/cynomolgus monkey B7-H1/PD-L1.
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Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human, Cynomolgus Monkey

Specificity

Natural and recombinant human B7-H1/PD-L1. This assay also recognizes natural cynomolgus monkey B7-H1/PD-L1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Sample Type	Mean (pg/mL)	Range (pg/mL)
Human Serum (n=36)	62.5	44.5-106
Cynomolgus Monkey Serum (n=10)	461	248-953
Human EDTA plasma (n=36)	56.2	39.1-108
Cynomolgus monkey EDTA plasma (n=10)	202	89.1-497
Human heparin plasma (n=36)	52.2	33.4-110

Sample Type	Mean of Detectable (pg/mL)	Range (pg/mL)
Human Urine (n=10)	34.5	ND-34.5

ND=Non-detectable

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	260	458	884	244	466	878
Standard Deviation	4.25	6.83	14.7	15.9	25.8	66.4
CV%	1.6	1.5	1.7	6.5	5.5	7.6

Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	257	490	934	249	469	898
Standard Deviation	3.75	7.01	12.7	11.1	21.7	37.7
CV%	1.5	1.4	1.4	4.5	4.6	4.2

Recovery for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

The recovery of human/cynomolgus monkey B7-H1/PD-L1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	105	97-112
DYC002 (1X) (n=1)	96	90-100
EDTA Plasma (n=4)	90	79-100
Heparin Plasma (n=4)	85	76-95
Serum (n=4)	89	81-100
Urine (n=4)	109	100-116

Linearity

Scientific Data Images for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve

Human PD-L1/B7-H1 ELISA Urine Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

Additional PD-L1/B7-H1 Products

Product Documents for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

For research use only

Citations
Reviews

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.