Human Cystatin C Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DSCTC0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL), Human Milk (50 µL)
Sensitivity
0.227 ng/mL
Assay Range
3.12-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human Cystatin C Quantikine ELISA Kit
The Quantikine Human Cystatin C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cystatin C in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Cystatin C and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cystatin C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Cystatin C.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Cystatin C
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for
the presence of human Cystatin C in this assay. No medical histories were available for the
donors used in this study.
Cell Culture Supernates:
ND=Non-detectable
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=36) | 792 | 553-1257 | 161 |
| EDTA plasma (n=36) | 774 | 560-1173 | 155 |
| Heparin plasma (n=36) | 786 | 524-1284 | 177 |
| Saliva (n=11) | 1259 | 103-3184 | 1077 |
| Urine (n=12) | 62.9 | 12.6-188 | 43.8 |
Cell Culture Supernates:
Human peripheral blood cells (1 x 106
cells/mL) were cultured in DMEM supplemented with
5% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and
100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with
10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for levels of
human Cystatin C.
| Condition | Day 1 (ng/mL) | Day 5 (ng/mL) |
| Unstimulated | ND | ND |
| Stimulated | ND | 16 |
IMR-90 human lung fibroblast cells were cultured in MEM supplemented with 10% fetal bovine
serum, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 0.1 mM sodium
pyruvate. An aliquot of the cell culture supernate was assayed for human Cystatin C and
measured 23.7 ng/mL.
MCF-7 human breast cancer cells were grown in DMEM/F12 media supplemented with
10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin
sulfate. An aliquot of the cell culture supernate was assayed for human Cystatin C and
measured 47.2 ng/mL.
HepG2 hepatocellular carcinoma cells were grown in DMEM supplemented with 10% fetal
bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An
aliquot of the cell culture supernate was assayed for human Cystatin C and measured
264 ng/mL.
Human Milk - Two human milk samples were assayed for human Cystatin C and measured
1456 ng/mL and 2431 ng/mL, respectively.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 16.2 | 29.9 | 52.6 | 17.2 | 30.8 | 60.9 |
| Standard Deviation | 1.07 | 0.92 | 2.41 | 1.21 | 1.53 | 3.60 |
| CV% | 6.6 | 3.1 | 4.6 | 7.0 | 5.0 | 5.9 |
Recovery for Human Cystatin C Quantikine ELISA Kit
The recovery of Cystatin C spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 88-102 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Cystatin C were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Cystatin C Quantikine ELISA Kit
Human Cystatin C ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cystatin C
Alternate Names
ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Gene Symbol
CST3
Additional Cystatin C Products
Product Documents for Human Cystatin C Quantikine ELISA Kit
Product Specific Notices for Human Cystatin C Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.