Human Cytochrome c Quantikine ELISA Kit

Name: Human Cytochrome c Quantikine ELISA Kit
Brand: R&D Systems
SKU: DCTC0

R&D Systems, part of Bio-Techne | Catalog # DCTC0

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Lysates (20 µL), Subcellular Fractions (100 µL)

Sensitivity

0.31 ng/mL

Assay Range

0.625-20 ng/mL (Cell Lysates, Subcellular Fractions)

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Product Summary for Human Cytochrome c Quantikine ELISA Kit

The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural human Cytochrome c

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with other substances.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Lysates, Subcellular Fractions

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	3.64	7.31	10.7	3.54	6.74	10.2
Standard Deviation	0.15	0.30	0.42	0.18	0.34	0.51
CV%	4.1	4.1	3.9	5.1	5.0	5.0

Linearity

To assess the linearity of the assay, six cell lysates containing high concentrations of Cytochrome c were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human Cytochrome c Quantikine ELISA Kit

Human Cytochrome c ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cytochrome c

Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol