Human DLL1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DLL10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL), Human Milk (50 µL)
Sensitivity
17.4 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Product Summary for Human DLL1 Quantikine ELISA Kit
The Quantikine Human DLL1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DLL1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human DLL1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human DLL1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DLL1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human DLL10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 638 | 1468 | 2934 | 592 | 1340 | 2714 |
| Standard Deviation | 16.0 | 38.1 | 76.1 | 42.4 | 85.7 | 189 |
| CV% | 2.5 | 2.6 | 2.6 | 7.2 | 6.4 | 7.0 |
Recovery for Human DLL1 Quantikine ELISA Kit
The recovery of DLL1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 96-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of DLL1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human DLL1 Quantikine ELISA Kit
Human DLL1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: DLL1
Long Name
Delta-like 1
Alternate Names
Delta 1
Gene Symbol
DLL1
Additional DLL1 Products
Product Documents for Human DLL1 Quantikine ELISA Kit
Product Specific Notices for Human DLL1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.