Human Endostatin Quantikine ELISA Kit

Name: Human Endostatin Quantikine ELISA Kit
Brand: R&D Systems
SKU: DNST0

R&D Systems, part of Bio-Techne | Catalog # DNST0

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL)

Sensitivity

0.063 ng/mL

Assay Range

0.31-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

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Product Summary for Human Endostatin Quantikine ELISA Kit

The Quantikine Human Endostatin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Endostatin in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human Endostatin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Endostatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Endostatin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human Endostatin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	0.70	2.02	4.14	0.76	2.13	4.21
Standard Deviation	0.05	0.12	0.15	0.06	0.13	0.24
CV%	6.9	6.0	3.6	7.9	6.1	5.7

Recovery for Human Endostatin Quantikine ELISA Kit

The recovery of Endostatin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	91-114
EDTA Plasma (n=4)	100	92-110
Heparin Plasma (n=4)	98	86-108
Serum (n=4)	100	93-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Endostatin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human Endostatin Quantikine ELISA Kit

Human Endostatin ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endostatin

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.

Alternate Names

COL18A1

Entrez Gene IDs

80781 (Human); 12822 (Mouse)

Gene Symbol