Human FABP2/I-FABP Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DFBP20
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
6.21 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human FABP2/I-FABP Quantikine ELISA Kit
The Quantikine Human FABP2/I-FABP Immunoassay is a 4.5 hour solid phase ELISA designed to measure FABP2 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains E.
coli-expressed recombinant human FABP2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FABP2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human FABP2 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human FABP2.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human FABP2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the
presence of human FABP2 in this assay. No medical histories were available for the donors used
in this study.
ND=Non-detectable
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=36) | 1015 | 389-2129 | 455 |
| EDTA plasma | 952 | 377-2049 | 444 |
| Heparin plasma (n=36) | 797 | 230-1800 | 381 |
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Urine (n=10) | 184 | 90 | ND-632 |
Cell Culture Supernates - Human peripheral blood leukocytes (PBL) were cultured in
RPMI 1640 and supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were then
cultured unstimulated or stimulated with 10 μg/mL PHA for 1 or 6 days. Aliquots of the cell
culture supernates were removed and assayed for levels of human FABP2. No detectable levels
were observed.
Cell Lysates - LoVo human colorectal adenocarcinoma cells were cultured in Kaighn's
supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin,
100 μg/mL streptomycin sulfate, and 1.5 g/L sodium bicarbonate and grown until confluent.
An aliquot of the cell lysate was removed, assayed for human FABP2, and measured
82.3 pg/mg.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 77.5 | 207 | 425 | 80.5 | 216 | 446 |
| Standard Deviation | 3.14 | 7.24 | 12.4 | 8.94 | 17.4 | 26.6 |
| CV% | 4.1 | 3.5 | 2.9 | 11.1 | 8.1 | 6.0 |
Recovery for Human FABP2/I-FABP Quantikine ELISA Kit
The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 96-104 |
| Cell Lysis Buffer (n=2) | 83 | 81-85 |
| EDTA Plasma (n=4) | 97 | 85-102 |
| Heparin Plasma (n=4) | 95 | 96-104 |
| Serum (n=4) | 101 | 97-106 |
| Urine (n=4) | 90 | 85-95 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FABP2/I-FABP
FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.
Long Name
Fatty Acid-Binding Protein 2/Intestinal FABP
Alternate Names
I-FABP, IFABP, Intestinal FABP
Gene Symbol
FABP2
Additional FABP2/I-FABP Products
Product Documents for Human FABP2/I-FABP Quantikine ELISA Kit
Product Specific Notices for Human FABP2/I-FABP Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.