Human FOLR1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DFLR10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (10 µL), Urine (10 µL), Human Milk (10 µL)
Sensitivity
14.9 pg/mL
Assay Range
46.9-3000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human FOLR1 Quantikine ELISA Kit
The Quantikine Human FOLR1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FOLR1 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human FOLR1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FOLR1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FOLR1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human FOLR1. This assay recognizes recombinant human FOLR1 in the presence of folic acid.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 377 | 1024 | 1922 | 390 | 1050 | 1973 |
| Standard Deviation | 9.28 | 19.8 | 29.5 | 20.3 | 64.0 | 135 |
| CV% | 2.5 | 1.9 | 1.5 | 5.2 | 6.1 | 6.9 |
Recovery for Human FOLR1 Quantikine ELISA Kit
The recovery of FOLR1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 94-107 |
| EDTA Plasma (n=4) | 95 | 84-106 |
| Heparin Plasma (n=4) | 96 | 82-105 |
| Serum (n=4) | 96 | 84-107 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of FOLR1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human FOLR1 Quantikine ELISA Kit
Human FOLR1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37-42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83 percent amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up-regulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk.
Long Name
Folate Receptor 1
Alternate Names
FBP, Folbp1, FR-alpha, MOv18
Gene Symbol
FOLR1
Additional FOLR1 Products
Product Documents for Human FOLR1 Quantikine ELISA Kit
Product Specific Notices for Human FOLR1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.